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amaxa nucleofector ii machine (program t-030)  (Lonza)


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    Structured Review

    Lonza amaxa nucleofector ii machine (program t-030)
    Amaxa Nucleofector Ii Machine (Program T 030), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofector+ii+machine/4d+nucleofector/pm39096891-61-18-24
    Average 90 stars, based on 1 article reviews
    amaxa nucleofector ii machine (program t-030) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Vimentin coordinates protein turnover at the aggresome during neural stem cell quiescence exit
    Article Snippet: .. Transfections Electroporations were performed on 4 million trypsinized NSCs resuspended in Mouse Nucleofector solution (Lonza VPG-1004) with 7 μg of Endotoxin-free DNA using the Mouse Neural Stem cell program in the Nucleofector II machine (Lonza). .. Plasmids generated in this study have been deposited to Addgene under catalog numbers listed in the “ .” All other resources used for this study are available through the Lead Contact, Darcie L. Moore ( ude.csiw@eroom.eicrad ) at reasonable request.

    Article Title: Viral reprogramming of host transcription initiation
    Article Snippet: EBV+ Burkitt's Lymphoma lines, Mutu I (obtained from the laboratory of Samuel H Speck) and Akata (obtained from Kenzo Takada), and EBV- Burkitt's Lymphoma line, DG75 (ATCC, cat no. CRL-2625) were cultured in RPMI (Fisher Scientific, cat no. SH30027) supplemented with 10% FBS (ThermoFisher Scientific, cat no. 10437) and 1% Pen/Strep (Fisher Scientific, cat no. 15140-122) in an incubator set to 37°C with 5% CO 2 . .. Three million Mutu or DG75 cells were transfected using an Amaxa Nucleofector II machine (Lonza). .. CMVp-GFP (0.3 μg) and SV40p-Zta (or SV40p-Cntl) or pLVX-Rta (or pLVX-Cntl) (2.7 μg) were added to each transfection reaction, along with 100 μl of Amaxa Cell Line Nucleofector Kit R (Lonza, cat no. VCA-1001).

    Electroporation:

    Article Title: Humanized cell line
    Article Snippet: The samples were examined by using Zeiss fluorescence microscopy (model Imager Z1; Carl Zeiss). .. Electroporation was performed using the AMAXA Nucleofector II machine (Lonza) according to the manufacturer's instructions. ..

    other:

    Article Title: Expression of the multidrug transporter P-glycoprotein is inversely related to that of apoptosis-associated endogenous TRAIL
    Article Snippet: 6 × 10 5 HeLa MDR-Off, KB-8-5-11 and KB-C1 cells were used for each point transfection through Amaxa® Cell Line Nucleofector® Kit R and the protocol was performed as recommended by the manufacturer.



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